The following procedure is recommended for decontaminating spills of agents used at BSL-2.

1. Wear gloves and a laboratory coat or gown. Heavyweight, puncture-resistant utility gloves, such as those used for housecleaning and dishwashing, are recommended.
   
   
2. Do not handle sharps with the hands. Clean up broken glass or other sharp objects with sheets of cardboard or other rigid, disposable material. If a broom and dustpan are used, they must be decontaminated later.
   
   
3. Avoid generating aerosols by sweeping.
   
   
4. Absorb the spill. Most disinfectants are less effective in the presence of high concentrations of protein, so absorb the bulk of the liquid before applying disinfectants. Use disposable absorbent material such as paper towels. After absorption of the liquid, dispose of all contaminated materials as waste.
   
   
5. Clean the spill site of all visible spilled material using an aqueous detergent solution (e.g., any household detergent). Absorb the bulk of the liquid to prevent dilution of the disinfectant.
   
   
6. Disinfect the spill site using an appropriate disinfectant, such as a household bleach solution. Flood the spill site or wipe it down with disposable towels soaked in the disinfectant.
   
   
7. Absorb the disinfectant or allow it to dry.
   
   
8. Rinse the spill site with water.
   
   
9. Dispose of all contaminated materials properly. Place them in a biohazard bag or other leakproof, labeled biohazard container for sterilization.