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Frequently Asked Question - Biological Safety
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Comparison
of Biological Safety Cabinets
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Type
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Face velocity (lfpm)
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Airflow Pattern
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Radionuclides/
Toxic Chemicals
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Biosafety Level(s)
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Product Protection
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| Class I* open front |
75
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In at front; rear and top through HEPA filter |
No
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2,3
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No
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| Class II Type A |
75
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70% recirculated through HEPA; exhaust through HEPA |
No
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2,3
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Yes
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| Type B1 |
100
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30% recirculated through HEPA; exhaust via HEPA
and hard ducted |
Yes (Low levels
or volatility)
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2,3
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Yes
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| Type B2 |
100
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No recirculation; total exhaust via HEPA and hard
ducted |
Yes
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2,3
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Yes
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| Type B3 |
100
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Same as IIA, but plena under negative pressure to
room and exhaust air is ducted |
Yes
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2,3
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Yes
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| Class III |
NA
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Supply air inlets and exhaust through 2 HEPA filters |
Yes
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3,4
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Yes
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* Glove panels may be added and will increase
face velocity to 150 lfpm; gloves may be added with an inlet
air pressure release that will allow work with chemicals/radionuclides.
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