A spill that is confined within a biological safety cabinet generally presents little or no hazard to personnel in the area. However, chemical disinfection procedures are to be initiated at once while the cabinet continues to operate. The disinfectant shall be one that is active against the organisms of potential hazard. Flammable liquids, such as ethanol or isopropanol, shall not be used, even if effective, because of the fire hazard of generating dangerous vapor concentrations within the cabinet that could be ignited by an electrical spark or other source.

Spray or wipe the walls, work surfaces, and equipment with the chosen disinfectant. Allow the disinfectant to remain on the surface for the appropriate contact time

Minimize the generation of aerosols and use sufficient disinfectant to ensure that drain pans and catch basins below the work surface contain disinfectant. The front exhaust shall also be wiped and the disinfectant drained into a container.